Polysome profiling and cancer

Author: akop

August undefined, 2024

WebA complex interplay between mRNA translation and cellular respiration has been recently unveiled, but its regulation in humans is poorly characterized in either health or disease. Cancer cells radically reshape both biosynthetic and bioenergetic WebSep 18, 2024 · Polysome profiling analysis of the early passage Eμ-Myc lymphoma “CX” cell lines and investigating potential roles of RAPGEF3 (EPAC1) and RAPGEF4 (EPAC2) in human blood cancers A. Enrichment analysis by GeneGO MetaCore ® of the polysomal RNA-seq data comparing CX-5461-resistant (CX) and drug-naïve cells (CTRL; n = 3; false discovery …

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WebThe structure of proline prevents it from adopting an optimal position for rapid protein synthesis. Poly-proline-tract (PPT) associated ribosomal stalling is resolved by highly conserved eIF5A, the only protein to contain the amino acid hypusine. We WebWilcoxon tests were performed to compare the difference between groups for each subunit. (E) Polysome profiling by sucrose-gradient-based centrifugation showing the changes in abundance of ribosomal components (40S, 60S, 80S monosome and polysomes) in A549 cells treated by specific siRNAs targeting eS8/RPS8, eS25/RPS25, uL5/RPL11 or … fnf henry stickman

Polysome Profiling Analysis of mRNA and Associated Proteins …

WebFeb 2, 2024 · The development and utilization of the polysome profiling technique for oocytes and zygotes have made the identification of actively translating mRNAs during MZT possible . Recently, an oocyte-specific (Zp3-Cre) RiboTag strategy has been used to characterize the dynamics of mRNA translation during oocyte maturation, which revealed … WebUsing polysome profiling, we show that HCMV presides over the cellular translational landscape, selectively accessing the host genome to extend its own coding capacity and regulate virus replication. WebI lead a multi-disciplinary research team composed of research professionals and trainees (i.e. students, postdocs) at different career stages with broad expertise in molecular biology, biochemistry, genomics and bio-informatics. We are primarily focused on studying the biological functions and mechanisms of intracellular RNA transport, as well as their … green unclassified banner

Polysome Fractionation and Analysis of Mammalian …

Analysis of translation using polysome profiling Nucleic Acids ...

WebI am processing samples for polysome profiling, and I've been getting great profiles for the primary cancer cell lines I've been working, but I have had trouble with the mouse embryonic fibroblast ... WebJan 23, 2015 · Modelska et al. carried out an important large-scale study to determine the clinical significance of expression of eIF4A1 and its modulators in breast cancer by … green uncharted island mapWebOct 7, 2016 · The polysome profile of fertilized embryos and the corresponding RNA profile of the 15–40% sucrose gradient separated on an agarose gel are shown in Figure 3B. In the first fractions, only low molecular weight RNAs were visible, then fractions containing only 40S subunits were isolated as shown by the presence of the 18S rRNA band, followed by … fnf henry stickmin 2.0

"WebROYGBV colors (with dashed lines as boundaries) indicate the polysome fractions that were combined before 3′-seq. (C,E) 3′-seq from polysome profiling from 24-h PHS testes, plotting relative level of the long 3′ UTR isoform detected across the different polysome fractions (in each column) for genes that undergo the stage-specific 3′ UTR shortening due to APA (in … " - Polysome profiling and cancer

Polysome profiling and cancer

Analysis of mRNA Translation by Polysome Profiling

WebApr 8, 2024 · Polysome profiling is a powerful method to study protein translation regulation. It allows to measure the translational status of individual mRNAs or examine … WebMar 1, 2024 · PROFILE With 20+ years of experience gained in academia and industry, I intersect biotechnology, molecular medicine, immunology and data science to make precision medicine for cancer, autoimmune conditions and rare diseases a reality. Critical thinking combined with a hands-on self-starter attitude was my key to become an …

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WebDec 23, 2024 · Moreover, by combining polysome profiling with CAGE-seq, we determine the translational efficiency for each 5′ transcript isoform and demonstrate that highly … WebPolysome profiling is a technique in molecular biology that is used to study the association of mRNAs with ribosomes. It is important to note that this technique is different from ribosome profiling. Both techniques have been …

WebPolysome-profiling revealed that one third of protein-coding mRNAs expressed in primary mouse macrophages are differentially translated upon infection with L. donovani promastigotes or ... Rapamycin can stimulate HSV1 replication in cancer cells, but active-site dual mTORC1 and mTORC2 (mammalian target of rapamycin complex 1 and 2 ... WebMiR-210 is induced by hypoxia and plays different roles in the development of certain cancers. However, little is known about its role in pancreatic cancer (PC). This study aimed to explore the induction and modulation of PC by miR-210 and its potential molecular targets. Methods.

WebDec 9, 2015 · Dynamic & enthusiastic Medical Researcher highly regarded for utilizing strong analytical skills and extensive laboratory experience to further advance within neuroscience and conduct cutting-edge cancer biology research. Strong background in molecular and cell biology, advanced microscopy, human/mice genetics, and bioinformatics. Broad … WebMay 17, 2014 · Genome-wide data obtained using polysome profiling is highly reproducible. MCF7 cells were treated as in Figure 1. Cytosolic and polysome-associated RNA (>3 ribosomes) was extracted from 4 ...

WebHere, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions ... Girault I et al (2014) eIF4F is a nexus of resistance …

WebRibosome profiling is a method based on deep sequencing of ribosome-protected mRNA fragments. Ribosome profiling, also known as Ribo-Seq (ribosome sequencing) or ART-Seq (active mRNA translation sequencing), provides a “snapshot” of all the ribosomes active in a cell at a specific time point. This information can help researchers determine ... green unc hatWeb(A) Heat map of distribution in the polysome gradient based on 3′-seq of 199 of the short 3′ UTR mRNA isoforms that predominantly comigrated with fractions lighter than the 80S monosome at the 48-h PHS time point from Figure 4 F. (B) Heat map of distribution in the polysome fractions from 72-h PHS testes of the same 199 short 3′ UTR mRNA isoforms … fnf henry stickmin chromatic scaleWebMay 15, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating … green uncle chipsWebMay 17, 2014 · An example of applying the polysome fractionation method to investigate the role of mTOR signaling in mediating the effects of insulin on mRNA translation is presented. To this end, MCF7 human breast cancer cells were maintained in low serum and then stimulated with insulin alone or in combination with the active-site mTOR inhibitor Torin1. green umpire shirtsWebSep 16, 2024 · Polysome profiling of cells treated with three hypothetical translation inhibitors is shown in the plots below. These three inhibitors are: (i) ... The cancer cells differentiated into a mesenchymal phenotype and grew in fresh culture medium containing inhibitors of epithelial-to-mesenchymal transition (EMT). green unclassified stickersWebMay 12, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating the response of glioblastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes of a set of human glioblastoma stem-like cell (GSC) lines. fnf henry stickmin expandedWebApr 8, 2024 · Recent studies demonstrated that protein translation dysregulation is associated with cancer, metabolic, neurodegenerative and other human diseases. Polysome profiling is a powerful method to study protein translation regulation. It allows to measure the translational status of individual mRNAs or examine translation on a genome-wide … fnf henry stickmin mod full week 3.0